Comparative genomic hybridization with CentoArray®
Comparative genomic hybridization is a molecular cytogenetic method for analyzing copy number variations. CENTOGENE‘s genome-wide array based solutions enable detection of known and novel structural aberrations such as copy number variations (CNVs), chromosomal imbalances, regions exhibiting loss/absence of heterozygosity (LOH), uniparental isodisomy (UDP) and even low-level mosaicism.
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Your advantages with CentoArray®
The major advantage of genomic arrays in detecting structural aberrations is that they can provide information on thousands of targets in a single experiment that can resolve any copy number changes ranging from gene, chromosome to genome level. Typically, high number of probes in the coding region of genes backed by backbone probes in the non-coding regions enables detection of not only previously described but also novel alterations in regions of interest.
Array CGH allows us to quickly and efficiently determine the relative abundance of nucleic acid sequences in the target sample. The method utilizes a large number of short stretches of synthetic DNA that probe for various genomic loci dispersed throughout the genome. Target DNA sample is labeled and hybridized with the DNA probes spotted on a solid array surface. Based on the relative amount of probe hybridized to each target spot, information is gained about the abundance of specific nucleic acid sequences within the sample.
CentoArray® offers you
- High resolution of chromosomal aberrations at the genome level
- Short turnaround time (15 business days)
- Highest performance with a wide range of sample types including formalin fixed paraffin embedded (FFPE) specimen
- Offered at an attractive price, CentoArray® is a cost effective alternative to MLPA and qPCR when analyzing multiple genes simultaneously
CentoArrayCyto® - CENTOGENE’s genome-wide array based solution
With markers targeted at both polymorphic and non-polymorphic regions spread across the genome, analysis of multiple genes associated with wide ranging phenotypes can be performed in a single assay.
CENTOGENE’s fully automated library preparation platform for CentoArrayCyto® reduces the variability between samples and provides high quality consistent data suitable for diagnostic applications.
- ≥ 2µg DNA, or
- 1ml of blood, or
- 1 CentoCard® (fully spotted), or
- at least 10 FFPE section of thickness 5-10 µm, or equivalent
CentoArrayCyto® - Key features
- Combines copy number markers with SNP markers at medium to high density to provide the
highest resolution and broadest coverage
- Reliably detects copy number changes across the genome with a resolution down to 25kb
- Confidently detects the presence of mosaicism down to 30%
- Compatible with a wide range of samples including blood, DNA, fresh and frozen tissues, amniocytes, bone marrow aspirate and even formalin fixed paraffin embedded (FFPE) samples
- An impressive TAT of 15 business days
- If an aberration is detected, we automatically validate this in the index using a second method (MLPA or qPCR) without additional costs.
CentoArrayCyto® is available in HD and 750K format. The key features of the different formats are described below to allow you to choose the best array for your specific need.
|CentoArrayCyto® HD||CentoArrayCyto® 750K|
|Features||High density (HD) genome wide array with highest resolution currently available||Medium density cost effective array to determine the chromosomal abnormalities|
|Total markers||2.6 Million||750,000|
|Resolution of CNVs detection||>25kb for copy number loss; |
>200kb for copy number gain
|>100kb for copy number loss; |
>200kb for copy number gain
|Detection of Mosaicism||Yes, >30%||Yes, >30%|
When to recommend CentoArrayCyto®
- As a 1st step analysis for cases of mental retardation and/or multiple malformations given that a considerable number of chromosomal rearrangements and CNVs have been implicated in such disorders
- In conjunction with whole exome sequencing to complement SNV with CNV detection. CentoArrayCyto® can be ordered either as a step-wise analysis with WES or as part of an attractive combined WES package
- CNV screening for large NGS panels if sequencing results are negative and single exon resolution analysis is not available
- For deletion/duplication analysis of extremely large genes where gross deletions involving large segment of gene, flanking intergenic regions or neighboring genes are frequently reported
- To diagnose uniparental iso-disomy
- For detection of mosaicism
- This analysis can be done as a prenatal testing. In this case, trio analysis of the index and the parents is highly recommended.