CENTOGENE’s genome-wide array based solutions enable detection of known and novel structural aberrations such as copy number variations (CNVs), chromosomal imbalances, regions exhibiting loss of heterozygosity (LOH), uniparental isodisomy (UDP) and even low-level mosaicism.
With markers targeted at both polymorphic and non-polymorphic regions spread across the genome, analysis of multiple genes associated with wide ranging phenotypes can be performed in a single assay.
CENTOGENE’s fully automated library preparation platform for CentoArrayCyto™ reduces the variability
between samples and provides high quality consistent data suitable for diagnostic applications.
CentoArrayCyto™ is available in HD and 750K format. The key features of the different formats are described below to allow you to choose the best array for your specific need.
CentoArrayCyto™ - HD
CentoArrayCyto™ - 750K
High density (HD) genome wide array with highest resolution currently
Medium density cost effective array to determine the chromosomal
Resolution of CNVs detection
>25kb for copy number loss;
>100kb for copy number loss;
Detection of Mosaicism
CentoArrayCyto™ - Key Features
- Combines copy number markers with SNP markers at extremely high density to provide the
highest resolution and broadest coverage
- Detects copy number changes across the genome with a resolution down to 25kb
- Confidently detects the presence of mosaicism down to 30%
- Compatible with a wide range of samples including blood, DNA, fresh and frozen tissues, amniocytes, bone marrow aspirate and even formalin fixed paraffin embedded (FFPE) samples
- An impressive TAT of 15 working days
- CentoArrayCyto™ can also be performed as a prenatal diagnostics. In prenatal cases, we highly recommend performing trio analysis of the index and the parents
- If a clinically relevant Class 1 or Class 2 aberration is detected, we validate this in the index using a second method (MLPA or qPCR) without additional costs. In addition, we highly recommend testing the parents, which may be performed using CentoArrayCyto™ or MLPA or qPCR. Choosing the proper method depends on the identified aberration(s)
- ≥ 2µg DNA, or
- 1ml of blood, or
- 1 CentoCard® (fully spotted), or
- at least 10 FFPE section of thickness 5-10 µm, or equivalent.
NOTE: for somatic mutation analysis we recommend CentoArrayOnco™.
For specific request to analyze tumor material with CentoArrayCyto™, please refer to the sample requirements for CentoArrayOnco™
When to recommend CentoArrayCyto™
- As a 1st step analysis for cases of mental retardation and/or multiple malformations given that a
considerable number of chromosomal rearrangements and CNVs have been implicated in such
- In conjunction with whole exome/genome sequencing, in order to complement SNV with CNV detection. CentoArrayCyto™ can be ordered either as a step-wise analysis with WES/WGS or as part of an
attractive combined WES/WGS package
- CNV screening for large NGS panels if sequencing results are negative and single exon resolution
analysis is not available
- For deletion/ duplication analysis of extremely large genes where gross deletions involving large
segment of gene, flanking intergenic regions or neighboring genes are frequently reported
- To diagnose uniparental iso-disomy
- This analysis can be done as a prenatal testing. In this case, trio analysis of the index and the parents is highly recommended.
Please contact our specialist medical team for more specific information. They are more than happy to
assist you to quickly determine the best solution for your specific needs.
Please note: Prenatal testing is not offered in the US.